Many aspects of animal development including fertilization as well as organ formation and function are dependent upon the dynamic release of calcium (Ca(2+)) ions. Although the controlled release and/or accumulation of Ca(2+) ions has been extensively studied, how the release dynamics produce a specific biological output in embryonic development is less clear. We will briefly summarize Ca(2+) sources, highlight data on endogenous Ca(2+) release in vertebrate embryos relevant to body plan formation and cell movement, and integrate pharmacological and molecular-genetic studies to lend insight into the signalling pathways involved. Finally, based on in vivo imaging in zebrafish genetic mutants, we will put forward the model that distinct Ca(2+) release dynamics lead to antagonism of the developmentally important Wnt/beta-catenin signalling pathway, while sustained Ca(2+) release modulates cell polarization or directed migration.