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Nat Methods. 2008 Feb;5(2):167-9. doi: 10.1038/nmeth.1173. Epub 2008 Jan 13.

Plasmid-chromosome shuffling for non-deletion alleles in yeast.

Author information

1
Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA.

Abstract

Here we describe a facile plasmid-chromosome shuffling technique for generating and analyzing non-deletion alleles in the yeast Saccharomyces cerevisiae. This technique takes advantage of an existing set of genome-wide haploid-convertible heterozygous diploid yeast knockout mutants. This simple method will facilitate characterization of essential gene functions and genome-wide investigation of protein structure-function relationships.

PMID:
18193055
DOI:
10.1038/nmeth.1173
[Indexed for MEDLINE]
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