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J Neurosci Methods. 2008 Mar 30;169(1):1-7. doi: 10.1016/j.jneumeth.2007.11.011. Epub 2007 Nov 28.

Stable in vivo imaging of densely populated glia, axons and blood vessels in the mouse spinal cord using two-photon microscopy.

Author information

1
Department of Pharmacology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA. davalos@ucsd.edu

Abstract

In vivo imaging has revolutionized our understanding of biological processes in brain physiology and pathology. However, breathing-induced movement artifacts have impeded the application of this powerful tool in studies of the living spinal cord. Here we describe in detail a method to image stably and repetitively, using two-photon microscopy, the living spinal tissue in mice with dense fluorescent cells or axons, without the need for animal intubation or image post-processing. This simplified technique can greatly expand the application of in vivo imaging to study spinal cord injury, regeneration, physiology and disease.

PMID:
18192022
PMCID:
PMC2647134
DOI:
10.1016/j.jneumeth.2007.11.011
[Indexed for MEDLINE]
Free PMC Article

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