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Hum Immunol. 2007 Dec;68(12):947-9. doi: 10.1016/j.humimm.2007.10.013. Epub 2007 Nov 26.

DP reactive antibody in a zero mismatch renal transplant pair.

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1
Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA. svaidya@utmb.edu

Abstract

This study describes molecular basis for positive B-cell flow crossmatch in a zero mismatch (0MM) transplant pair. Our end-stage renal disease patient was B-cell flow crossmatch positive with a 0MM deceased donor. DNA from the donor and patient were further typed by a high-resolution method. The patient's sera were tested for anti-HLA reactivity by single antigen bead using a Luminex platform. The patient and donor were found to be HLA identical except for a single DP allele MM (DPB1*0601). As there was no single antigen bead coated with DPB*0601, analysis of the amino acid residues of reactive and nonreactive DPB1 alleles was conducted. The results showed that all reactive alleles carried the amino acid D-E at residue 55 and 56 of DPB1. The MM allele DPB1*0601 also carries the DE 55-56 epitope. We conclude that positive B-cell flow crossmatch was likely the result of single MM in the DP locus.

PMID:
18191721
DOI:
10.1016/j.humimm.2007.10.013
[Indexed for MEDLINE]
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