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Cell. 2008 Jan 11;132(1):113-24. doi: 10.1016/j.cell.2007.11.042.

PTRF-Cavin, a conserved cytoplasmic protein required for caveola formation and function.

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1
Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia.

Abstract

Caveolae are abundant cell-surface organelles involved in lipid regulation and endocytosis. We used comparative proteomics to identify PTRF (also called Cav-p60, Cavin) as a putative caveolar coat protein. PTRF-Cavin selectively associates with mature caveolae at the plasma membrane but not Golgi-localized caveolin. In prostate cancer PC3 cells, and during development of zebrafish notochord, lack of PTRF-Cavin expression correlates with lack of caveolae, and caveolin resides on flat plasma membrane. Expression of PTRF-Cavin in PC3 cells is sufficient to cause formation of caveolae. Knockdown of PTRF-Cavin reduces caveolae density, both in mammalian cells and in the zebrafish. Caveolin remains on the plasma membrane in PTRF-Cavin knockdown cells but exhibits increased lateral mobility and accelerated lysosomal degradation. We conclude that PTRF-Cavin is required for caveola formation and sequestration of mobile caveolin into immobile caveolae.

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PMID:
18191225
PMCID:
PMC2265257
DOI:
10.1016/j.cell.2007.11.042
[Indexed for MEDLINE]
Free PMC Article
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