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J Proteome Res. 2008 Feb;7(2):780-5. doi: 10.1021/pr0705340. Epub 2008 Jan 12.

Heat-shock response in Arabidopsis thaliana explored by multiplexed quantitative proteomics using differential metabolic labeling.

Author information

1
The BioCentre, The University of Reading, Whiteknights, Post Office Box 221, Reading RG6 6AS, United Kingdom. n.m.palmblad@lumc.nl

Abstract

We have developed a general method for multiplexed quantitative proteomics using differential metabolic stable isotope labeling and mass spectrometry. The method was successfully used to study the dynamics of heat-shock response in Arabidopsis thaliana. A number of known heat-shock proteins were confirmed, and some proteins not previously associated with heat shock were discovered. The method is applicable in stable isotope labeling and allows for high degrees of multiplexing.

PMID:
18189342
DOI:
10.1021/pr0705340
[Indexed for MEDLINE]

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