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Dev Comp Immunol. 2008;32(7):814-24. doi: 10.1016/j.dci.2007.12.001. Epub 2007 Dec 26.

Expression and characterization of two STAT isoforms from Sf9 cells.

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1
Department of Food and Nutrition, Hung-Kuang University, Taichung, Taiwan.

Abstract

In invertebrates, the JAK-STAT signaling pathway is involved in the anti-bacterial response and is part of an anti-viral response in Drosophila. In this study, we show that two STAT transcripts are generated by alternative splicing and encode two isoforms of Sf-STAT with different C-terminal ends. These two isoforms were produced and purified using the recombinant baculovirus technology. Both purified isoforms showed similar DNA-binding activity and displayed weak but significant transactivation potential toward a Drosophila promoter that contained a STAT-binding motif. No significant activation of the Sf-STAT protein in Sf9 cells was found by infection with baculovirus AcMNPV.

PMID:
18187191
DOI:
10.1016/j.dci.2007.12.001
[Indexed for MEDLINE]
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