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BMC Bioinformatics. 2008 Jan 10;9:13. doi: 10.1186/1471-2105-9-13.

FCI: an R-based algorithm for evaluating uncertainty of absolute real-time PCR quantification.

Author information

1
National Genetics Reference Laboratory (Manchester), St, Mary's Hospital, Manchester, UK. paolo.verderio@istitutotumori.mi.it

Abstract

BACKGROUND:

FCI is an R code for analyzing data from real-time PCR experiments. This algorithm estimates standard curve features as well as nucleic acid concentrations and confidence intervals according to Fieller's theorem.

RESULTS:

In order to describe the features of FCI four situations were selected from real data collected during an international external quality assessment program for quantitative assays based on real-time PCR. The code generates a diagnostic figure suitable for assessing the quality of the quantification process.

CONCLUSION:

We have provided a freeware programme using this algorithm specifically designed to increase the information content of the real-time PCR assay.

PMID:
18186926
PMCID:
PMC2245902
DOI:
10.1186/1471-2105-9-13
[Indexed for MEDLINE]
Free PMC Article
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