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J Med Assoc Thai. 2007 Nov;90(11):2422-8.

Myristica fragrans Houtt. methanolic extract induces apoptosis in a human leukemia cell line through SIRT1 mRNA downregulation.

Author information

1
Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. chintana_chula@yahoo.co.th

Abstract

BACKGROUND:

Myristica fragrans Houtt. (nutmeg) contains antibacterial, antiviral and anti-cancer activities. However the mechanisms underlying those activities have not been clearly explained.

OBJECTIVE:

To study the effect of Myristica fragrans Houtt. methanolic extract on Jurkat human leukemia T cell line.

MATERIAL AND METHOD:

Methanol extract of Myristica fragrans Houtt. (Myristicaceae) was used to study the effect on Jurkat cell metabolic activity using an MTT assay and on apoptosis using annexin V staining. Expression of SIRT1 gene was determined by RT-PCR.

RESULTS:

At the concentrations 50 and 100 ig/mL, the methanol extract of Myristica fragrans Houtt significantly inhibited Jurkat cell proliferation and induced apoptosis as detected by annexin V staining. Downregulation of SIRT1 mRNA expression in Jurkat cells was observed even when the amount of methanol extract was 10 microg/mL.

CONCLUSION:

Methanol extract of Myristica fragrans Houtt induced apoptosis of Jurkat leukemia T cell line in a mechanisms involving SIRTI mRNA downregulation.

PMID:
18181330
[Indexed for MEDLINE]

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