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J Bacteriol. 2008 Mar;190(6):2217-20. doi: 10.1128/JB.01778-07. Epub 2008 Jan 4.

Identification of the archaeal alg7 gene homolog (encoding N-acetylglucosamine-1-phosphate transferase) of the N-linked glycosylation system by cross-domain complementation in Saccharomyces cerevisiae.

Author information

1
Institute for Virology, AG Parasitologie BMFZ, Philipps-University Marburg, Hans-Meerwein-Str. 2, 35043 Marburg, Germany.

Abstract

The Mv1751 gene product is thought to catalyze the first step in the N-glycosylation pathway in Methanococcus voltae. Here, we show that a conditional lethal mutation in the alg7 gene (N-acetylglucosamine-1-phosphate transferase) in Saccharomyces cerevisiae was successfully complemented with Mv1751, highlighting a rare case of cross-domain complementation.

PMID:
18178736
PMCID:
PMC2258894
DOI:
10.1128/JB.01778-07
[Indexed for MEDLINE]
Free PMC Article

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