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J Acquir Immune Defic Syndr. 2007 Nov 1;46(3):261-7.

Sequence variation occurs in CD4 epitopes during early HIV infection.

Author information

1
Partners AIDS Research Center, Massachusetts General Hospital, Boston, MA 02114, USA.

Abstract

OBJECTIVE:

To determine whether viral sequence variation occurs in HIV-specific CD4 epitopes during early HIV infection.

METHODS:

Gag, Nef, and integrase (Int) sequences were obtained from the plasma of 7 subjects identified during acute HIV-1 infection. Changes in the viral sequence were determined based on comparison of sequences obtained at 2 time points during early infection. Peptide-specific CD4+ T-cell responses were measured at matched time points using interferon-gamma enzyme-linked immunosorbent spot assays to identify CD4 epitopes.

RESULTS:

An average of 4 mutations were identified per subject. The majority of the mutations were nonsynonymous and resulted in a total of 6 amino acid changes in Gag, 7 changes in Nef, and 6 changes and a deletion in Int. Half of the sequence changes were within recognized CD4 epitopes. Mutations within CD4 epitopes were coincident with changes in the peptide-specific CD4 response.

CONCLUSION:

These data indicate that sequence variation occurs within recognized CD4 epitopes during early HIV infection. Furthermore, it suggests that mutations within HIV-specific CD4 epitopes may affect T helper cell function.

PMID:
18167642
DOI:
10.1097/qai.0b013e3181514427
[Indexed for MEDLINE]

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