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BMC Genomics. 2007 Dec 31;8:479. doi: 10.1186/1471-2164-8-479.

MacroH2A1 knockdown effects on the Peg3 imprinted domain.

Author information

1
Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, 70803, USA. jhchoo@lsu.edu

Abstract

BACKGROUND:

MacroH2A1 is a histone variant that is closely associated with the repressed regions of chromosomes. A recent study revealed that this histone variant is highly enriched in the inactive alleles of Imprinting Control Regions (ICRs).

RESULTS:

The current study investigates the potential roles of macroH2A1 in genomic imprinting by lowering the cellular levels of the macroH2A1 protein. RNAi-based macroH2A1 knockdown experiments in Neuro2A cells changed the expression levels of a subset of genes, including Peg3 and Usp29 of the Peg3 domain. The expression of these genes was down-regulated, rather than up-regulated, in response to reduced protein levels of the potential repressor macroH2A1. This down-regulation was not accompanied with changes in the DNA methylation status of the Peg3 domain.

CONCLUSION:

MacroH2A1 may not function as a transcriptional repressor for this domain, but that macroH2A1 may participate in the heterochromatin formation with functions yet to be discovered.

PMID:
18166131
PMCID:
PMC2241636
DOI:
10.1186/1471-2164-8-479
[Indexed for MEDLINE]
Free PMC Article
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