Format

Send to

Choose Destination
Nat Methods. 2008 Jan;5(1):53-5. Epub 2007 Dec 23.

GraFix: sample preparation for single-particle electron cryomicroscopy.

Author information

1
Department of Cellular Biochemsitry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany. bkastne@gwdg.de

Abstract

We developed a method, named GraFix, that considerably improves sample quality for structure determination by single-particle electron cryomicroscopy (cryo-EM). GraFix uses a glycerol gradient centrifugation step in which the complexes are centrifuged into an increasing concentration of a chemical fixation reagent to prevent aggregation and to stabilize individual macromolecules. The method can be used to prepare samples for negative-stain, cryo-negative-stain and, particularly, unstained cryo-EM.

PMID:
18157137
DOI:
10.1038/nmeth1139
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center