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Appl Environ Microbiol. 2008 Feb;74(4):1294-8. Epub 2007 Dec 21.

Determination of glycosyltransferase specificities for the Escherichia coli O111 O antigen by a generic approach.

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1
School of Molecular and Microbial Biosciences, University of Sydney, Sydney, NSW 2006, Australia.

Abstract

We describe a bacterial strain developed to facilitate the determination of glycosyltransferase (GT) specificities for O antigens of known structure and gene cluster sequence. For proof of principle for the approach, the strain was used to determine the specificity of the Escherichia coli O111 O-antigen GT genes.

PMID:
18156323
PMCID:
PMC2258590
DOI:
10.1128/AEM.02660-07
[Indexed for MEDLINE]
Free PMC Article
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