Conduction velocity is regulated by sodium channel inactivation in unmyelinated axons innervating the rat cranial meninges

J Physiol. 2008 Feb 15;586(4):1089-103. doi: 10.1113/jphysiol.2007.145383. Epub 2007 Dec 20.

Abstract

Axonal conduction velocity varies according to the level of preceding impulse activity. In unmyelinated axons this typically results in a slowing of conduction velocity and a parallel increase in threshold. It is currently held that Na(+)-K(+)-ATPase-dependent axonal hyperpolarization is responsible for this slowing but this has long been equivocal. We therefore examined conduction velocity changes during repetitive activation of single unmyelinated axons innervating the rat cranial meninges. In direct contradiction to the currently accepted postulate, Na(+)-K(+)-ATPase blockade actually enhanced activity-induced conduction velocity slowing, while the degree of velocity slowing was curtailed in the presence of lidocaine (10-300 microm) and carbamazepine (30-500 microm) but not tetrodotoxin (TTX, 10-80 nm). This suggests that a change in the number of available sodium channels is the most prominent factor responsible for activity-induced changes in conduction velocity in unmyelinated axons. At moderate stimulus frequencies, axonal conduction velocity is determined by an interaction between residual sodium channel inactivation following each impulse and the retrieval of channels from inactivation by a concomitant Na(+)-K(+)-ATPase-mediated hyperpolarization. Since the process is primarily dependent upon sodium channel availability, tracking conduction velocity provides a means of accessing relative changes in the excitability of nociceptive neurons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carbamazepine / pharmacology
  • Cyanides / pharmacology
  • Enzyme Inhibitors / pharmacology
  • Female
  • Lidocaine / pharmacology
  • Lithium / metabolism
  • Male
  • Meninges / cytology
  • Meninges / metabolism*
  • Nerve Fibers, Unmyelinated / metabolism*
  • Neural Conduction / physiology*
  • Neurons, Afferent / metabolism*
  • Ouabain / pharmacology
  • Potassium / metabolism
  • Rats
  • Rats, Wistar
  • Sodium / metabolism
  • Sodium Channels / drug effects
  • Sodium Channels / metabolism*
  • Sodium-Potassium-Exchanging ATPase / metabolism
  • Synaptic Transmission / physiology
  • Tetrodotoxin / pharmacology

Substances

  • Cyanides
  • Enzyme Inhibitors
  • Sodium Channels
  • Carbamazepine
  • Tetrodotoxin
  • Ouabain
  • Lidocaine
  • Lithium
  • Sodium
  • Sodium-Potassium-Exchanging ATPase
  • Potassium