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Proteomics. 2008 Jan;8(1):32-6.

Post alignment clustering procedure for comparative quantitative proteomics LC-MS data.

Author information

1
Plant Research International, Wageningen-UR, Wageningen, The Netherlands.

Abstract

Comparative LC-MS is a powerful method for detailed quantitative comparison of complex protein mixtures. Dedicated software is required for detection, matching, and alignment of peaks in multiple LC-MS datasets. However, retention time shifts, saturation effects, limitations of experimental accuracy, and possible occurrence of split peaks make it difficult for software to perfectly match all chromatograms. We describe a procedure to assess the above problems and show that dataset quality can be enhanced with the aid of cluster analysis.

PMID:
18095361
DOI:
10.1002/pmic.200700707
[Indexed for MEDLINE]

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