Format

Send to

Choose Destination
Proteomics. 2008 Jan;8(1):21-7.

Proteomic profiling using mass spectrometry--does normalising by total ion current potentially mask some biological differences?

Author information

1
Clinical and Biomedical Proteomics Group, Cancer Research UK Clinical Centre, Leeds Institute of Molecular Medicine, St. James's University Hospital, Leeds, UK.

Abstract

Normalisation of data to minimise the impact of technical variation on comparative sample analysis is often carried out. Using SELDI data as a model, we have examined the effects of normalisation by TIC which is commonly used for MS data. Significant intergroup differences in normalisation factor were found for serum profiles which could not be explained by experimental factors, implying that normalisation by TIC may in some situations also normalise biological differences and should be systematically evaluated.

PMID:
18095358
DOI:
10.1002/pmic.200700598
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center