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Arch Microbiol. 2008 May;189(5):519-24. Epub 2007 Dec 19.

Azospirillum brasilense Sp7 produces an outer-membrane lectin that specifically binds to surface-exposed extracellular polysaccharide produced by the bacterium.

Author information

1
Cátedra de Bioquímica, Departamento de Biociencias, Facultad de Química, CC 1157, Montevideo, Uruguay.

Abstract

Azospirillum sp promotes the growth of many important crop plants. We demonstrated lectin binding activity in outer-membrane protein extracts of A. brasilense Sp7 by hemagglutination assays. The lectin specifically recognised the exopolysaccharide (EPS) produced by aggregated cells. Affinity chromatography using EPS-Sepharose was used to identify a 67 kDa outer-membrane lectin (OML) that recognised a binding region in the extracellular polysaccharide. Results show the specific recognition and binding between EPS and OML. The potential relationship between cell-to-cell aggregation and the OML-EPS interaction is discussed.

PMID:
18094958
DOI:
10.1007/s00203-007-0343-5
[Indexed for MEDLINE]
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