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Acta Crystallogr D Biol Crystallogr. 2008 Jan;64(Pt 1):70-5. Epub 2007 Dec 5.

Combining X-ray and electron-microscopy data to solve crystal structures.

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1
Laboratoire de Microscopie Electronique Structurale, Institut de Biologie Structurale Jean-Pierre Ebel, 41 Rue Jules Horowitz, F-38027 Grenoble, France. jorge.navaza@ibs.fr

Abstract

Low-resolution electron-microscopy reconstructions can be used as search models in molecular replacement or may be combined with existing monomeric structures in order to produce multimeric models suitable for molecular replacement. The technique is described in the case of viral and subviral particles as well as in the case of oligomeric proteins.

PMID:
18094469
PMCID:
PMC2394810
DOI:
10.1107/S0907444907053334
[Indexed for MEDLINE]
Free PMC Article
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