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Nat Struct Mol Biol. 2008 Jan;15(1):106-8. Epub 2007 Dec 16.

Subunit organization and functional transitions in Ci-VSP.

Author information

1
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.

Abstract

Voltage-sensing domains (VSDs) confer voltage dependence on effector domains of membrane proteins. Ion channels use four VSDs to control a gate in the pore domain, but in the recently discovered phosphatase Ci-VSP, the number of subunits has been unknown. Using single-molecule microscopy to count subunits and voltage clamp fluorometry to detect structural dynamics, we found Ci-VSP to be a monomer, which operates independently, but nevertheless undergoes multiple voltage-dependent conformational transitions.

PMID:
18084307
DOI:
10.1038/nsmb1320
[Indexed for MEDLINE]

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