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Nat Methods. 2008 Jan;5(1):87-93. Epub 2007 Dec 16.

Tracking transmitter-gated P2X cation channel activation in vitro and in vivo.

Author information

1
Department of Physiology, University of California Los Angeles, 10833 LeConte Avenue, Los Angeles, California 90095, USA.

Abstract

We present a noninvasive approach to track activation of ATP-gated P2X receptors and potentially other transmitter-gated cation channels that show calcium fluxes. We genetically engineered rat P2X receptors to carry calcium sensors near the channel pore and tested this as a reporter for P2X(2) receptor opening. The method has several advantages over previous attempts to image P2X channel activation by fluorescence resonance energy transfer (FRET): notably, it reports channel opening rather than a conformation change in the receptor protein. Our FRET-based imaging approach can be used as a general method to track, in real time, the location, regional expression variation, mobility and activation of transmitter-gated P2X channels in living neurons in vitro and in vivo. This approach should help to determine when, where and how different receptors are activated during physiological processes.

PMID:
18084300
PMCID:
PMC3523716
DOI:
10.1038/nmeth1144
[Indexed for MEDLINE]
Free PMC Article

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