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Nat Methods. 2008 Jan;5(1):49-51. Epub 2007 Dec 16.

Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster.

Author information

1
Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02175, USA.

Abstract

The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site-driven RNA interference constructs have been inserted into the genome randomly using P-element-mediated transformation, which can result in false negatives due to variable expression. To avoid this problem, we have developed a transgenic RNA interference vector based on the phiC31 site-specific integration method.

PMID:
18084299
PMCID:
PMC2290002
DOI:
10.1038/nmeth1146
[Indexed for MEDLINE]
Free PMC Article

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