Format

Send to

Choose Destination
Mol Biochem Parasitol. 2008 Feb;157(2):187-92. Epub 2007 Nov 9.

The canine hookworm genome: analysis and classification of Ancylostoma caninum survey sequences.

Author information

1
Department of Genetics, Genome Sequencing Center, Washington University School of Medicine, St Louis, MO 63108, USA.

Abstract

Hookworms infect nearly a billion people. The Ancylostoma caninum hookworm of canids is a model for studying human infections and information from its genome coupled with functional genomics and proteomics can accelerate progress towards hookworm control. As a step towards a full-scale A. caninum genome project, we generated 104,000 genome survey sequences (GSSs) and determined the genome size of the canine hookworm. GSSs assembled into 57.6 Mb of unique sequence from a genome that we estimate by flow cytometry of isolated nuclei to be 347 +/- 1.2 Mb, substantially larger than other Rhabditina species. Gene finding identified 5538 genes in the GSS assembly, for a total of 9113 non-redundant A. caninum genes when EST sequences are also considered. Functional classifications of many of the 70% of genes with homology to genes in other species are provided based on gene ontology and KEGG associations and secreted and membrane-bound proteins are also identified.

PMID:
18082904
PMCID:
PMC2265417
DOI:
10.1016/j.molbiopara.2007.11.001
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center