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Comparison between chicken and rabbit antibody based particle enhanced cystatin C reagents for immunoturbidimetry.

Author information

1
Department of Medical Sciences, University Hospital, Uppsala, Sweden.

Abstract

We have compared three commercial particle enhanced cystatin C reagents. One of the reagents utilizes chicken antibodies and the other two reagents are rabbit antibody based. We show that the chicken antibody based reagent yields a higher delta absorbance when reacting with the antigen. IgY coupled to latex particles show a strong scatter response even at high antigen concentrations in contrast to the steep decline in scatter previously reported for IgY antibodies in solution. The reagent also showed a low CV for duplicate samples. Laying hens thus seems as an interesting source of antibodies for particle-enhanced immunoassays.

PMID:
18080876
DOI:
10.1080/15321810701734644
[Indexed for MEDLINE]

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