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Biosci Biotechnol Biochem. 2007 Dec;71(12):2943-51. Epub 2007 Dec 7.

A new method for the modification of fibroin heavy chain protein in the transgenic silkworm.

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1
National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan. kojikei@affrc.go.jp

Abstract

We constructed a new plasmid vector for the production of a modified silk fibroin heavy chain protein (H-chain) in the transgenic silkworm. The plasmid (pHC-null) contained the promoter and the 3' region of a gene encoding the H-chain and the coding regions for the N-terminal domain and the C-terminal domain of the H-chain. For the model protein, we cloned a foreign gene that encoded EGFP between the N-terminal domain and the C-terminal domain in pHC-null and generated transgenic silkworms that produced a modified H-chain, HC-EGFP. Transgenic silkworms produced HC-EGFP in the posterior part of silk gland cells, secreted it into the lumen of the gland, and produced a cocoon with HC-EGFP as part of the fibroin proteins. N-terminal sequencing of HC-EGFP localized the signal sequence cleavage site to between positions A((21)) and N((22)). These results indicate that our new plasmid successfully produced the modified H-chain in a transgenic silkworm.

PMID:
18071257
[Indexed for MEDLINE]
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