Foxb1-driven Cre expression in somites and the neuroepithelium of diencephalon, brainstem, and spinal cord

Genesis. 2007 Dec;45(12):781-7. doi: 10.1002/dvg.20356.

Abstract

We have knocked-in Cre-IRES-EGFP in the Foxb1 locus by homologous recombination in embryonic stem cells. We removed the PGK-neo cassette (which was flanked by FRT sequences) by crossing with the FLPeR deleter mouse. The Foxb1(Cre) line showed Cre recombinase activity as well as EGFP fluorescence reproducing Foxb1 expression accurately. By crossing Foxb1(Cre) mice with the ROSA26R and Z/AP mouse reporter lines we have been able to trace the lineage of Foxb1-expressing cells. Early transient expression of Foxb1 in the paraxial mesoderm translates into labeling of the somites. In the central nervous system (CNS), the Foxb1 lineage includes the thalamus and mammillary body (hypothalamus), brainstem, and the ventral spinal cord and floor plate.

Publication types

  • Research Support, Non-U.S. Gov't
  • Technical Report

MeSH terms

  • Animals
  • Brain Stem / embryology
  • Brain Stem / metabolism
  • Central Nervous System / embryology*
  • Central Nervous System / metabolism
  • Diencephalon / embryology
  • Diencephalon / metabolism
  • Embryonic Stem Cells / metabolism
  • Forkhead Transcription Factors / genetics*
  • Green Fluorescent Proteins / genetics
  • Integrases / analysis
  • Integrases / genetics*
  • Integrases / metabolism
  • Mice
  • Mice, Transgenic
  • Mutagenesis, Insertional / methods*
  • Neurons / metabolism
  • Recombination, Genetic
  • Somites / embryology
  • Somites / metabolism
  • Spinal Cord / embryology
  • Spinal Cord / metabolism

Substances

  • Forkhead Transcription Factors
  • Foxb1 protein, mouse
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins
  • Cre recombinase
  • Integrases