Send to

Choose Destination
See comment in PubMed Commons below
Opt Lett. 2000 May 1;25(9):634-6.

Theoretical study of detection of a dipole emitter through an objective with high numerical aperture.


The problem of detecting electric dipole emitters (such as a fluorescing molecule) through a microscope objective with a high numerical aperture is considered. Exact vector-wave-optics results of the field distribution of the electromagnetic field in image space are derived. On the basis of these results, the collection-efficiency function (CEF) is calculated. This calculation is compared with a semigeometric approximation of the CEF, for which a new analytical formula is presented. The derived results are important for applications such as fluorescence correlation spectroscopy and single-molecule fluorescence detection with a confocal microscope.

PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Loading ...
    Support Center