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Appl Microbiol Biotechnol. 2008 Feb;78(1):157-63. Epub 2007 Dec 1.

Substrate specificity of Stenotrophomonas nitritireducens in the hydroxylation of unsaturated fatty acid.

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  • 1Department of Bioscience and Biotechnology, Konkuk University, Seoul, 143-701, South Korea.

Abstract

An isolated bacterium that converted unsaturated fatty acids to hydroxy fatty acids was identified as Stenotrophomonas nitritireducens by API analysis, cellular fatty acids compositions, sequencing the full 16S ribosomal ribonucleic acid, and evaluating its nitrite reduction ability. S. nitritireducens has unique regio-specificity for C16 and C18 cis-9 unsaturated fatty acids. These fatty acids are converted to their 10-hydroxy fatty acids without detectable byproducts. Among the cis-9-unsaturated fatty acids, S. nitritireducens showed the highest specificity for linoleic acid. The cells converted 20 mM linoleic acid to 13.5 mM 10-hydroxy-12(Z)-octadecenoic acid at 30 degrees C and pH 7.5 with a yield of 67.5% (mol/mol).

PMID:
18060400
DOI:
10.1007/s00253-007-1280-6
[PubMed - indexed for MEDLINE]
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