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Genome Biol. 2007;8 Suppl 1:S3.

Transposon mediated transgenesis in a marine invertebrate chordate: Ciona intestinalis.

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1
Shimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka, 415-0025, Japan. sasakura@kurofune.shimoda.tsukuba.ac.jp

Abstract

Achievement of transposon mediated germline transgenesis in a basal chordate, Ciona intestinalis, is discussed. A Tc1/mariner superfamily transposon, Minos, has excision and transposition activities in Ciona. Minos enables the creation of stable transgenic lines, enhancer detection, and insertional mutagenesis.

PMID:
18047695
PMCID:
PMC2106840
DOI:
10.1186/gb-2007-8-s1-s3
[Indexed for MEDLINE]
Free PMC Article
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