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Protein Pept Lett. 2007;14(9):894-902.

Protein-protein and protein-ligand interactions studied by electrospray-ionization mass spectrometry.

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Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milan, Italy.


Preservation of non-covalent interactions in biopolymer mass spectrometry offers new approaches to binding analysis. Recent work from our laboratory is reviewed here and discussed with reference to recent literature in the field. Three issues are considered in particular: hydrophobically stabilized complexes, pH-dependent transitions, and linked protein-ligand and protein-protein binding equilibria.

[Indexed for MEDLINE]

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