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BMC Dev Biol. 2007 Nov 23;7:129.

Gene array identification of Ipf1/Pdx1-/- regulated genes in pancreatic progenitor cells.

Author information

1
Umeå Center for Molecular Medicine, Umeå University, SE-901 87 Umeå, Sweden. per.svensson@ucmm.umu.se

Abstract

BACKGROUND:

The homeodomain transcription factor IPF1/PDX1 exerts a dual role in the pancreas; Ipf1/Pdx1 global null mutants fail to develop a pancreas whereas conditional inactivation of Ipf1/Pdx1 in beta-cells leads to impaired beta-cell function and diabetes. Although several putative target genes have been linked to the beta-cell function of Ipf1/Pdx1, relatively little is known with respect to genes regulated by IPF1/PDX1 in early pancreatic progenitor cells.

RESULTS:

Microarray analyses identified a total of 111 genes that were differentially expressed in e10.5 pancreatic buds of Ipf1/Pdx1-/- embryos. The expression of one of these, Spondin 1, which encodes an extracellular matrix protein, has not previously been described in the pancreas. Quantitative real-time RT-PCR analyses and immunohistochemical analyses also revealed that the expression of FgfR2IIIb, that encodes the receptor for FGF10, was down-regulated in Ipf1/Pdx1-/- pancreatic progenitor cells.

CONCLUSION:

This microarray analysis has identified a number of candidate genes that are differentially expressed in Ipf1/Pdx1-/- pancreatic buds. Several of the differentially expressed genes were known to be important for pancreatic progenitor cell proliferation and differentiation whereas others have not previously been associated with pancreatic development.

PMID:
18036209
PMCID:
PMC2212654
DOI:
10.1186/1471-213X-7-129
[Indexed for MEDLINE]
Free PMC Article
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