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Stem Cells. 2008 Feb;26(2):525-33. Epub 2007 Nov 21.

In vitro- and in vivo-induced transgene expression in human embryonic stem cells and derivatives.

Author information

1
WiCell Research Institute, Madison, Wisconsin, USA.

Abstract

The use of human embryonic stem cells (hESCs) as a research and therapeutic tool will be facilitated by conditional gene expression. Here, we report drug-induced transgene expression, both in vitro and in vivo, from a tet-on hESC line with >95% purity. Using green fluorescent protein as an indicator, we demonstrated that the tet-on system allowed a tight control of the gene expression in both undifferentiated hESCs and differentiated cells of the three germ layers. More importantly, after the cells were transplanted into animals, the gene expression remained to be regulated by an orally administered drug. These results provide a technical basis for regulation of gene expression in hESCs and derivatives in vitro and in vivo.

PMID:
18032700
PMCID:
PMC2707826
DOI:
10.1634/stemcells.2007-0710
[Indexed for MEDLINE]
Free PMC Article

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