Comparison of BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR versus the CHROMagar MRSA assay for screening patients for the presence of MRSA strains

J Clin Microbiol. 2008 Jan;46(1):350-1. doi: 10.1128/JCM.02130-07. Epub 2007 Nov 21.

Abstract

We compared the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) real-time PCR assay with the CHROMagar MRSA assay for the detection of MRSA in 286 nasal surveillance specimens. Compared with the CHROMagar MRSA assay, PCR had sensitivity, specificity, positive predictive value, and negative predictive values of 100%, 98.6%, 95.8%, and 100%, respectively. The mean PCR turnaround time was 14.5 h.

Publication types

  • Comparative Study

MeSH terms

  • Bacteriological Techniques / methods*
  • Carrier State / diagnosis
  • Carrier State / microbiology*
  • Culture Media / chemistry
  • Hospitals, Teaching
  • Humans
  • Methicillin Resistance*
  • Nose / microbiology
  • Polymerase Chain Reaction / methods*
  • Predictive Value of Tests
  • Sensitivity and Specificity
  • Staphylococcal Infections / diagnosis
  • Staphylococcal Infections / microbiology*
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / isolation & purification*
  • Time Factors

Substances

  • Culture Media