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Application of on-chip capillary electrophoresis to cell-free preparation of recombinant DNA.

Author information

1
Center for International Research on MicroMechatronics, Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan. nojima@iis.u-tokyo.ac.jp

Abstract

An on-chip capillary electrophoresis-based DNA collection was applied to the isolation of target DNA species from a DNA mixture generated by a polymerase chain reaction (PCR), whose starting material was a ligation mixture of an insert and an expression vector. The collected DNA was then amplified by PCR and properly worked as template DNA in a coupled cell-free transcription/translation system. These results demonstrateed that total operation in standard genetic engineering can be performed in a cell-free condition.

PMID:
18029599
DOI:
10.1093/nass/nrm044
[Indexed for MEDLINE]

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