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Anal Biochem. 2008 Feb 15;373(2):383-5. Epub 2007 Sep 29.

DNA digestion to deoxyribonucleoside: a simplified one-step procedure.

Author information

1
Biomedical Mass Spectrometry Laboratory, General Clinical Research Center, University of Florida, Gainesville, FL 32611, USA. epq@ufl.edu

Abstract

We present a simple and inexpensive 'one-step' protocol for the hydrolysis of DNA to deoxyribonucleosides. Unlike the older DNA hydrolysis protocol which is cumbersome and labor intensive, this new protocol is ideal for high-throughput assays and is suitable automation. Using this protocol we were able to hydrolyze several hundred samples within an 8-hour period. The new protocol is fully compatible with LC-MS/MS and gives similar recoveries for all five major deoxyribonucleosides when compared to the older protocol.

PMID:
18028864
PMCID:
PMC2239294
DOI:
10.1016/j.ab.2007.09.031
[Indexed for MEDLINE]
Free PMC Article

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