Format

Send to

Choose Destination
See comment in PubMed Commons below
Cell Host Microbe. 2007 Apr 19;1(2):95-107.

A type III secretion system in Vibrio cholerae translocates a formin/spire hybrid-like actin nucleator to promote intestinal colonization.

Author information

1
Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.

Abstract

We have previously characterized a non-O1, non-O139 Vibrio cholerae strain, AM-19226, that lacks the known virulence factors but contains components of a type III secretion system (T3SS). In this study, we demonstrated that the T3SS is functional and is required for intestinal colonization in the infant mouse model. We also identified VopF, which is conserved among T3SS-positive V. cholerae strains, as an effector containing both formin homology 1-like (FH1-like) and WASP homology 2 (WH2) domains. Translocation of VopF by V. cholerae or expression by transfection altered the actin cytoskeletal organization of the eukaryotic host cells. In vitro domain analysis indicated that both FH1-like and WH2 domains are required for actin nucleation and polymerization activity. These data correlate with in vivo data, suggesting that VopF-mediated alteration of actin polymerization homeostasis is required for efficient intestinal colonization by T3SS+V. cholerae in the infant mouse model.

PMID:
18005688
DOI:
10.1016/j.chom.2007.03.005
[Indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center