Spirolide toxin profile of Adriatic Alexandrium ostenfeldii cultures and structure elucidation of 27-hydroxy-13,19-didesmethyl spirolide C

J Nat Prod. 2007 Dec;70(12):1878-83. doi: 10.1021/np0703242. Epub 2007 Nov 16.

Abstract

This paper reports on the determination of the toxin profile of batch cultures of Alexandrium ostenfeldii established from water samples collected along the Emilia-Romagna coasts (Italy) in November 2003. Combined use of LC-MS/MS and 1D and 2D NMR techniques led us to identify 13,19-didesmethylspirolide C and 13-desmethylspirolide C as products of the alga and to highlight the occurrence of a new component, 27-hydroxy-13,19-didesmethylspirolide C, whose structure was fully elucidated by NMR. MS data for a number of minor spirolides are also reported.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Dinoflagellida / chemistry*
  • Italy
  • Marine Toxins / chemistry
  • Marine Toxins / pharmacokinetics*
  • Molecular Structure
  • Nuclear Magnetic Resonance, Biomolecular
  • Spiro Compounds / chemistry
  • Spiro Compounds / pharmacokinetics*

Substances

  • 27-hydroxy-13,19-didesmethyl spirolide C
  • Marine Toxins
  • Spiro Compounds
  • spirolide C