Format

Send to

Choose Destination
Mol Syst Biol. 2007;3:143. Epub 2007 Nov 13.

Accurate prediction of gene feedback circuit behavior from component properties.

Author information

1
Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.

Abstract

A basic assumption underlying synthetic biology is that analysis of genetic circuit elements, such as regulatory proteins and promoters, can be used to understand and predict the behavior of circuits containing those elements. To test this assumption, we used time-lapse fluorescence microscopy to quantitatively analyze two autoregulatory negative feedback circuits. By measuring the gene regulation functions of the corresponding repressor-promoter interactions, we accurately predicted the expression level of the autoregulatory feedback loops, in molecular units. This demonstration that quantitative characterization of regulatory elements can predict the behavior of genetic circuits supports a fundamental requirement of synthetic biology.

PMID:
18004276
PMCID:
PMC2132446
DOI:
10.1038/msb4100185
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center