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Histochem Cell Biol. 2008 Jan;129(1):33-43. Epub 2007 Nov 14.

Morphological and cytochemical determination of cell death by apoptosis.

Author information

1
Department of Pathology, University of Vermont, 89 Beaumont Avenue, Burlington, VT, 05405, USA. douglas.taatjes@uvm.edu

Abstract

Several modes of cell death are now recognized, including necrosis, apoptosis, and autophagy. Oftentimes the distinctions between these various modes may not be apparent, although the precise mode may be physiologically important. Accordingly, it is often desirable to be able to classify the mode of cell death. Apoptosis was originally defined by structural alterations in cells observable by transmitted light and electron microscopy. Today, a wide variety of imaging and cytochemical techniques are available for the investigation of apoptosis. This review will highlight many of these methods, and provide a critique on the advantages and disadvantages associated with them for the specific identification of apoptotic cells in culture and tissues.

PMID:
18000678
PMCID:
PMC2137940
DOI:
10.1007/s00418-007-0356-9
[Indexed for MEDLINE]
Free PMC Article

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