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BMC Genomics. 2007 Nov 9;8:411.

Ganoderma lucidum polysaccharides in human monocytic leukemia cells: from gene expression to network construction.

Author information

1
Department of Life Science, National Taiwan University, Taipei 106, Taiwan. jerekcheng@gmail.com

Abstract

BACKGROUND:

Ganoderma lucidum has been widely used as a herbal medicine for promoting health and longevity in China and other Asian countries. Polysaccharide extracts from Ganoderma lucidum have been reported to exhibit immuno-modulating and anti-tumor activities. In previous studies, F3, the active component of the polysaccharide extract, was found to activate various cytokines such as IL-1, IL-6, IL-12, and TNF-alpha. This gave rise to our investigation on how F3 stimulates immuno-modulating or anti-tumor effects in human leukemia THP-1 cells.

RESULTS:

Here, we integrated time-course DNA microarray analysis, quantitative PCR assays, and bioinformatics methods to study the F3-induced effects in THP-1 cells. Significantly disturbed pathways induced by F3 were identified with statistical analysis on microarray data. The apoptosis induction through the DR3 and DR4/5 death receptors was found to be one of the most significant pathways and play a key role in THP-1 cells after F3 treatment. Based on time-course gene expression measurements of the identified pathway, we reconstructed a plausible regulatory network of the involved genes using reverse-engineering computational approach.

CONCLUSION:

Our results showed that F3 may induce death receptor ligands to initiate signaling via receptor oligomerization, recruitment of specialized adaptor proteins and activation of caspase cascades.

PMID:
17996095
PMCID:
PMC2211495
DOI:
10.1186/1471-2164-8-411
[Indexed for MEDLINE]
Free PMC Article

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