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Platelet gel stimulates proliferation of human dermal fibroblasts in vitro.

Author information

1
Department for Blood Supply, Blood Transfusion Center of Slovenia, Slajmerjeva 6, 1000 Ljubljana, Slovenia. metka.krasna@ztm.si

Abstract

BACKGROUND:

Platelet gel is a source of many soluble proteins that can modulate tissue regeneration. Dermal fibroblasts play an important role in tissue remodeling and wound healing.

OBJECTIVE:

The study was performed to investigate the influence of platelet gel and its active ingredients on the proliferation of human dermal fibroblasts in vitro.

METHODS:

A fibroblast culture was established from a normal human skin biopsy. Platelet gel was prepared from platelet-rich plasma (PRP) following the addition of calcified thrombin solution. Cell proliferation was measured by MTT assay.

RESULTS:

We showed that platelet gel, PRP, and thrombin stimulate the proliferation of dermal fibroblasts, and that stimulation by PRP is dose dependent.

CONCLUSION:

Platelet gel can be used to treat chronic skin defects.

PMID:
17994170
[Indexed for MEDLINE]
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