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Nat Struct Mol Biol. 2007 Dec;14(12):1196-201. Epub 2007 Nov 11.

NC2 mobilizes TBP on core promoter TATA boxes.

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Department of Chemistry and Biochemistry and Center for Nanoscience (CeNS), Ludwig Maximilian University, Butenandtstr. 11, 81377 Munich, Germany.


The general transcription factors (GTFs) of eukaryotic RNA polymerase II, in a process facilitated by regulatory and accessory factors, target promoters through synergistic interactions with core elements. The specific binding of the TATA box-binding protein (TBP) to the TATA box has led to the assumption that GTFs recognize promoters directly, producing a preinitiation complex at a defined position. Using biochemical analysis as well as biophysical single-pair Förster resonance energy transfer, we now provide evidence that negative cofactor-2 (NC2) induces dynamic conformational changes in the TBP-DNA complex that allow it to escape and return to TATA-binding mode. This can lead to movement of TBP along the DNA away from TATA.

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