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Nat Methods. 2007 Dec;4(12):1003-5. Epub 2007 Nov 11.

Rapid control of protein level in the apicomplexan Toxoplasma gondii.

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1
Hygieneinstitut, Department of Parasitology, University Hospital Heidelberg, Im Neuenheimer Feld 324, D-69120 Heidelberg, Germany.

Erratum in

  • Nat Methods. 2008 Jan;5(1):113.

Abstract

Analysis of gene function in apicomplexan parasites is limited by the absence of reverse genetic tools that allow easy and rapid modulation of protein levels. The fusion of a ligand-controlled destabilization domain (ddFKBP) to a protein of interest enables rapid and reversible protein stabilization in T. gondii. This allows an efficient functional analysis of proteins that have a dual role during host cell invasion and/or intracellular growth of the parasite.

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PMID:
17994029
PMCID:
PMC2601725
DOI:
10.1038/nmeth1134
[Indexed for MEDLINE]
Free PMC Article
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