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J Wildl Dis. 2007 Oct;43(4):775-9.

Mycobacterium avium subspecies paratuberculosis in Bison (Bison bison) from Northern Canada.

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Department of Microbiology and Immunology, University of British Colombia, 6174 University Blvd., Vancouver, British Columbia V6T 1Z3, Canada.


Nested polymerase chain reaction (PCR) using the Mycobacterium avium subspecies paratuberculosis (Map)-specific region, locus 251, was used as a screening tool for the detection of Map DNA in fecal samples from northern Canadian bison herds. Further characterization of positive samples (26/835) was performed because Map DNA was found without signs of disease. Strain typing, using PCR-Restriction endonucleas assay (REA), was limited to two samples but revealed that the samples corresponded to a cattle-related strain and a sheep-related strain. Sequencing of part of the IS1311 region from the two samples revealed a unique three base-pair region, which is only found within the northern Canadian bison isolates.

[Indexed for MEDLINE]

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