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J Wildl Dis. 2007 Oct;43(4):775-9.

Mycobacterium avium subspecies paratuberculosis in Bison (Bison bison) from Northern Canada.

Author information

1
Department of Microbiology and Immunology, University of British Colombia, 6174 University Blvd., Vancouver, British Columbia V6T 1Z3, Canada.

Abstract

Nested polymerase chain reaction (PCR) using the Mycobacterium avium subspecies paratuberculosis (Map)-specific region, locus 251, was used as a screening tool for the detection of Map DNA in fecal samples from northern Canadian bison herds. Further characterization of positive samples (26/835) was performed because Map DNA was found without signs of disease. Strain typing, using PCR-Restriction endonucleas assay (REA), was limited to two samples but revealed that the samples corresponded to a cattle-related strain and a sheep-related strain. Sequencing of part of the IS1311 region from the two samples revealed a unique three base-pair region, which is only found within the northern Canadian bison isolates.

PMID:
17984279
DOI:
10.7589/0090-3558-43.4.775
[Indexed for MEDLINE]

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