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FEBS Lett. 2007 Nov 27;581(28):5469-74. Epub 2007 Nov 5.

Histological mapping of biochemical changes in solid tumors by FT-IR spectral imaging.

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University of Bordeaux 2, Centre National de la Recherche Scientifique, UMR 5084, 146 Rue Léo Saignat, F33076 Bordeaux, France.


Fourier-transform infrared (FT-IR) spectral imaging was used for analyzing biochemical changes in tumor cells. Metabolic parameters of human lung A549/8 adenocarcinoma and U87 glioma cells were compared under stress conditions in culture along with tumor progression after cell implantation onto the chick embryo chorio-allantoic membrane. In cell culture, glucose consumption and lactic acid release were higher in U87 cells. A549/8 cells were less sensitive to oxidative stress as observed through changes in fatty acyl chains. In vivo biochemical mapping of highly (U87) vs. poorly (A549/8) angiogenic tumors provided results comparable to culture models. Therefore, FT-IR imaging allows detecting subtle chemical changes in tumors, which might be useful for diagnosis.

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