Format

Send to

Choose Destination
Nat Methods. 2007 Dec;4(12):1011-3. Epub 2007 Nov 4.

Quantifying small numbers of antibodies with a 'near-universal' protein-DNA chimera.

Author information

1
Center for Quantitative Genome Function and Molecular Sciences Institute, 2168 Shattuck Avenue, Berkeley, California 94704, USA.

Abstract

We present general means to greatly increase the sensitivity of antibody-based assays. Augmentation relies on a 'tadpole' protein-DNA chimera whose protein moiety binds most classes of mammalian antibodies but not avian immunoglobulin Y (IgY). We used this tadpole in affinity capture assays followed by real-time PCR to quantify numerous molecules, including prostate-specific antigen (PSA) in human serum, with great sensitivity and accuracy.

PMID:
17982460
DOI:
10.1038/nmeth1127
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center