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FEBS Lett. 2007 Nov 27;581(28):5425-9. Epub 2007 Oct 30.

Locked 5Zs-biliverdin blocks the Meta-RA to Meta-RC transition in the functional cycle of bacteriophytochrome Agp1.

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Department of Physics, Freie Universit├Ąt Berlin, D-14195 Berlin, Germany.


The bacteriophytochrome Agp1 was reconstituted with a locked 5Zs-biliverdin in which the C(4)=C(5) and C(5)-C(6) bonds of the methine bridge between rings A and B are fixed in the Z and syn configuration/conformation, respectively. In Agp1-5Zs the photoconversion proceeds via the Lumi-R intermediate to Meta-R(A), but the following millisecond-transition to Meta-R(C) is blocked. Consistently, no transient proton release was detected. The photoconversion of Agp1-5Zs is apparently arrested in a Meta-R(A)-like intermediate, since the subsequent syn to anti rotation around the C(5)-C(6) bond is prevented by the lock. The Meta-R(A)-like photoproduct was characterized by its distinctive CD spectrum suggesting a reorientation of ring D.

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