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Biotechnol Lett. 2008 Mar;30(3):405-10. Epub 2007 Nov 1.

Development of 3-D nanofibrous fibroin scaffold with high porosity by electrospinning: implications for bone regeneration.

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1
Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul, Republic of Korea.

Abstract

We made a three-dimensional (3-D) nanofibrous fibroin scaffold (NFS) with high porosity (94%) and examined its feasibility in bone regeneration. Under scanning electron microscopy, MC3T3-E1 preosteoblasts on the scaffold showed more spread on the first day after seeding compared with a 2-D scaffold. MTT assay showed significantly increased proliferation in 3-D NFS compared with 2-D NFS 7 days after seeding (P < 0.05). Western immunoblotting for activated paxillin, FAK, AKT, C-Src, and ERK1/2 antibodies showed signals from the extracellular matrix were significantly increased in 3-D NFS. Newly developed 3-D electrospun NFS may be a good candidate for use in bone regeneration.

PMID:
17973083
DOI:
10.1007/s10529-007-9581-5
[Indexed for MEDLINE]
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