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Anal Bioanal Chem. 2008 Feb;390(3):841-6. Epub 2007 Oct 31.

Miniaturized planar lipid bilayer: increased stability, low electric noise and fast fluid perfusion.

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Jacobs University Bremen, Campus Ring 1, 28759, Bremen, Germany.


A microfluidic device was designed allowing the formation of a planar lipid bilayer across a micron-sized aperture in a glass slide sandwiched between two polydimethylsiloxane channel systems. By flushing giant unilamellar vesicles through a 500-microm-wide channel above the hole, we were able to form a planar lipid bilayer across the hole, resulting in a giga-seal. We demonstrate incorporation of biological nanopores into the bilayer. This miniaturized system offers noise recordings comparable to open head-stage noise (under 1 pA RMS at 10 kHz), fast precision perfusion on each side of the membrane and the use of nanoliter analyte volumes. This technique shows a promising potential for automation and parallelization of electrophysiological setups.

[Indexed for MEDLINE]

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