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Anticancer Res. 2007 Sep-Oct;27(5A):3235-43.

Altered gene expression by cisplatin in a human squamous cell lung carcinoma cell line.

Author information

1
Department of Respirology, Graduate School of Medicine, Chiba University, Chiba, Japan.

Abstract

BACKGROUND:

Substantial evidence has disclosed that some cytotoxic agents have complex activities in influencing signal transduction pathways in cells.

MATERIALS AND METHODS:

cDNA microarray analysis was performed after exposing a human squamous cell carcinoma cell line, RERF-LC-AI, to low-dose cisplatin for 5 days. Up-regulated gene expressions were suppressed by small interfering RNA to investigate phenotypic alteration of the cells.

RESULTS:

Among 30,000 genes screened, 42 genes showed increases or decreases in expression of more than 2-fold with cisplatin treatment. They included genes with functions involved in apoptosis, cell cycle regulation and DNA metabolism/repair. Suppression of the 5 most significantly altered genes by small interfering RNA resulted in partly reduced apoptosis without altering cytotoxicity of cisplatin.

CONCLUSION:

Besides direct cytotoxic effects on cells, cisplatin may have indirect effects involving drug resistance, and synergistic effects with other agents.

PMID:
17970066
[Indexed for MEDLINE]
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